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University of Wisconsin–Madison

Gene Expression Center

Agilent 2100 BioAnalyzer

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

Room 3225 Independent Access Policy (June 2020)

Please note: Currently, access to the equipment in Biotech Center room 3225 is limited to internal UWBC research groups.

You must notify us of your intent to use the equipment at least 24 hours prior. This allows us to ensure the room is adequately stocked with required supplies in order to maintain appropriate social distancing.

UWBC COVID Room 3225 entry Protocol (6-2-20)
 
Please e-mail gecinfo@biotech.wisc.edu to request access to the Google calendar for scheduling your usage.

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The Bioanalyzer utilizes 'Lab-on-a-Chip' technology to integrate sample preparation, fluid handling, separation, detection, and data analysis. Providing researchers with standardized methods and enhanced reproducibility. Each chip contains an interconnected set of gel-filled channels that allow for molecular sieving of nucleic acid and protein samples.

Quick and Easy Operation

*Simple chip preparation requires less than 20 minutes set-up time.
*Ready to use reagent kits.
*Only 1ul of sample required
*Only 30 minutes to run and analyze 11-12 samples.
*Data is displayed as a gel-image or electropherogram
*Automatically calculates fragment size, concentration, ribosomal RNA ratios,
*High detection sensitivity. More sensitive than a polyacrylamide gel stained with EtBr or  SYBR Gold.
*Built in quality control with custom array ladders.

 The Gene Expression Center stocks both the Agilent RNA Nano and Pico kits, as well as the DNA 1000 kit.


Independent Access

The Agilent 2100 Bioanalyzer is available for independent access once users have been satisfactorily trained by lab personnel.   The cost of training cost is $100/hour plus the cost of consumables, and a training session generally takes 1.5 to 2 hours.  Please read the Training Info and Training Form prior to training.  To schedule training, please contact the GEC with the completed Training Form.

Agilent Training Form_v3.3

Agilent Training Info

Agilent Chips Available UW Rate
External Rate
Agilent RNA6000 NanoChip $54
$84
Agilent RNA6000 PicoChip $58 $90
Agilent DNA 1000 Chip $56 $87

 
Price includes the Agilent Chip, consumables, reagents and RNA/DNA ladder.
Prices effective 4/1/2020

Gene Expression Center Agilent Scheduling and Use policy

Interested users may buy their own kits  and pay a $18 instrument access fee.

For more information visit the Agilent web site.

Or contact: gecinfo@biotech.wisc.edu

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.