In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.
QIAseq miRNA Library Kit
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA / Nextera XT (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
Agilent Sure Select Target Enrichment
We request all new users of our services please contact firstname.lastname@example.org to set up a meeting to discuss the proposed project.
To discuss a project or obtain a quote please e-mail email@example.com.
Please review our updated sample drop-off guidelines (effective 1/25/2021), revised to comply with COVID-19 safety precautions: GEC Sample Exchange Policy
Samples may be submitted on Tuesday and Thursday between 9-10AM by appointment.
Appointments may be made by e-mailing firstname.lastname@example.org.
All sample submissions must include completed electronic copies of both the RNA-Seq Submission Form and Sample Submission sheet. Sample submission guidelines are available in the Sample Submission sheet. If you have any questions please let us know.
DOCUMENTS FOR DOWNLOAD
Illumina RNA-seq Submission Form (Updated March 2021)
GEC Sample Submission Spreadsheet (Updated March 2021)
The UWBC Bioinformatics Resource Center offers data analysis support for all RNA Seq projects processed by the Gene Expression Center.
A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement:
"The author(s) utilized the University of Wisconsin – Madison Biotechnology Center’s Gene Expression Center Core Facility (Research Resource Identifier - RRID:SCR_017757) for RNA library preparation and the DNA Sequencing Facility (RRID:SCR_017759) for sequencing."
* RNA is verified for quantity/purity and quality on a NanoDrop One spectrophotometer and Agilent 2100 Bioanalyzer, respectively.
Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples) ≥ 100ng/uL
PicoChip (11 samples) ≤ 100ng/uL
Pricing listed above effective 4/1/2021.
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above. This surcharge is subject to change.
Illumina service pricing is broken into two components: library preparation and sequencing. Library preparation is charged on a per sample basis and varies by type. Sequencing charges are based upon sequencing type (i.e.shared 2x150bp, 1x100bp, etc.) and depth.
Prices are subject to change due to increasing reagent costs.