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University of Wisconsin–Madison

Gene Expression Center

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

RNA Library Preparations Services

In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.

The following RNA library preparation services are available:

QIAseq miRNA Library Kit
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA  / Nextera XT  (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
Agilent Sure Select Target Enrichment

We request all new users of our services please contact gecinfo@biotech.wisc.edu to set up a meeting to discuss the proposed project.

GETTING STARTED

To discuss a project or obtain a quote please e-mail gecinfo@biotech.wisc.edu.

Please review our updated sample drop-off guidelines (effective 1/25/2021), revised to comply with COVID-19 safety precautions: GEC Sample Exchange Policy

Sample Submission

Samples may be submitted on Tuesday and Thursday between 9-10AM by appointment.

Appointments may be made by e-mailing gecinfo@biotech.wisc.edu

All sample submissions must include completed electronic copies of both the RNA-Seq Submission Form and Sample Submission sheet.  Sample submission guidelines are available in the Sample Submission sheet.  If you have any questions please let us know.

DOCUMENTS FOR DOWNLOAD

Illumina RNA-seq Submission Form (Updated March 2021)

GEC Sample Submission Spreadsheet (Updated March 2021)

Data Analysis

The UWBC Bioinformatics Resource Center offers data analysis support for all RNA Seq projects processed by the Gene Expression Center.

Acknowledging University of Wisconsin-Madison Biotechnology Center

A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement:

"
The author(s) utilized the University of Wisconsin – Madison Biotechnology Center’s Gene Expression Center Core Facility (Research Resource Identifier - RRID:SCR_017757) for RNA library preparation and the DNA Sequencing Facility (RRID:SCR_017759) for sequencing."

    UW System
Rates

Non UW System Rates
Library Type  Details
Samples
1-48

Samples 
  49-96

Samples 
1-48

Samples
49-96

TruSeq Stranded mRNA   Sample 1
$1,545 $1,860
$2,352 $2,840
 
Each additional sample
$110 $110
$127 $127
TruSeq Stranded Total RNA (Gold)
Sample 1
 $1,610 $1,925
 $2,430 $2,920
  Each additional sample
  $175
$175
 $220 $220
    Samples 
1-24


 

Samples
1-24


QiaSeq miRNA
Sample 1
$1,350
  $2,075

  Each additional sample
$115

 $135
    Samples  1-48
Samples
  49-96

Samples
1-48
 
Samples 
  49-96

Takara SMARTer v4 / NexteraXT
Sample 1
$1,515
$1,800
 $2,300 $2,750
  Each additional sample
$125
$125
 $145  $145
Takara SMARTer Stranded Total RNA Pico v2 Mammalian

Sample 1

$1,360
 $1,585  $2,055 $2,420
  Each additional sample
$110
$110
$130
$130
RNA QC- Agilent & NanoDrop


     
RNA NanoChip (up to 12 samples)
$171     $264
 
RNA PicoChip (up to 11 samples)
$175
    $270
 
Small RNA (up to 11 samples)
 $190     $295
 
DNA1000 Chip (up to 12 samples)
$173

  $267
 
HS DNA Chip (up to 11 samples)
$201

   $311  

* RNA is verified for quantity/purity and quality on a NanoDrop One spectrophotometer and Agilent 2100 Bioanalyzer, respectively. 

Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples)  ≥ 100ng/uL
PicoChip (11 samples)  ≤ 100ng/uL


Pricing listed above effective 4/1/2021.

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above.  This surcharge is subject to change.

Illumina service pricing is broken into two components: library preparation and sequencing.  Library preparation is charged on a per sample basis and varies by type.  Sequencing charges are based upon sequencing type (i.e.shared  2x150bp, 1x100bp, etc.)  and depth. 

Prices are subject to change due to increasing reagent costs.

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.