In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.
QIAseq miRNA Library Kit
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA / Nextera XT (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
Agilent Sure Select Target Enrichment
We request all new users of our services please contact firstname.lastname@example.org to set up a meeting to discuss the proposed project.
To discuss a project or obtain a quote please e-mail email@example.com.
Please review our updated sample drop-off guidelines (effective 9/1/2020), revised to comply with COVID-19 safety precautions: GEC Sample Drop-off Policy
Please contact firstname.lastname@example.org to arrange all sample submissions.
All sample submissions must include an electronic (email@example.com) copy of both the RNA-Seq Submission Form and the RNA-Seq Submission Spreadsheet with each sample name, concentration and volume. Please see our sample drop-off policy above.
Illumina RNA-Seq Fillable Submission Form, August 2020
RNA Sample Submission Spreadsheet, April 2020
Support is available for projects that are run at UWBC facilities, and is provided for a fee by the UWBC Bioinformatics Resource Center. Terminals and software are available in our computer lab for clients of UWBC facilities who are internal to UW-Madison or the University of Wisconsin System who need to perform further analysis but lack the necessary hardware and software.
A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement: "The author(s) thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing Illumina RNA library preparation and the DNA Sequencing Facility sequencing services”.
* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively.
Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples) ≥ 100ng/uL
PicoChip (11 samples) ≤ 100ng/uL
Pricing listed above effective 4/1/2020.
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above. This surcharge is subject to change.
Illumina service pricing is broken into two components: library preparation and sequencing. Library preparation is charged on a per sample basis and varies by type. Sequencing charges are based upon sequencing type (i.e.shared 2x150bp, 1x100bp, etc.) and depth.
Prices are subject to change due to increasing reagent costs.