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University of Wisconsin–Madison

Gene Expression Center

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

RNA Library Preparations Services

In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.

The following RNA library preparation services are available:

QIAseq miRNA Library Kit
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA  / Nextera XT  (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
Agilent Sure Select Target Enrichment

We request all new users of our services please contact gecinfo@biotech.wisc.edu to set up a meeting to discuss the proposed project.

Getting Started

To discuss a project or obtain a quote please e-mail gecinfo@biotech.wisc.edu.

Please review our updated sample drop-off guidelines, revised June 2020 to comply with COVID-19 safety precautions: GEC Sample Drop-off Policy

Sample Submission

Please contact gecinfo@biotech.wisc.edu to arrange all sample submissions.
All sample submissions must include an electronic (gecinfo@biotech.wisc.edu) copy of both the RNA-Seq Submission Form and the RNA-Seq Submission Spreadsheet with each sample name, concentration and volume. Please see our sample drop-off policy above.

DOCUMENTS FOR DOWNLOAD

Illumina RNA-Seq Fillable Submission Form, June 2020

RNA Sample Submission Spreadsheet, April 2020

Bioinformatics

Support is available for projects that are run at UWBC facilities, and is provided for a fee by the UWBC Bioinformatics Resource Center. Terminals and software are available in our computer lab for clients of UWBC facilities who are internal to UW-Madison or the University of Wisconsin System who need to perform further analysis but lack the necessary hardware and software.

Acknowledging UWBC and the Gene Expression Center

A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement: "The author(s) thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing Illumina RNA library preparation and the DNA Sequencing Facility sequencing services”.

    UW System
Rates

Non UW System Rates
Library Type  Details
Samples
1-48

Samples 
  49-96

Samples 
1-48

Samples
49-96

TruSeq Stranded mRNA   Sample 1
$1,545 $1,860
$2,352 $2,840
 
Each additional sample
$110 $110
$127 $127
TruSeq Stranded Total RNA Sample 1
 $1,610 $1,925
 $2,430 $2,920
  Each additional sample
  $175
$175
 $220 $220
    Samples 
1-24


 

Samples
1-24


QiaSeq miRNA
Sample 1
$1,315
  $1,995

  Each additional sample
$115

 $135
    Samples  1-48
Samples
  49-96

Samples
1-48
 
Samples 
  49-96

Takara SMARTer v4 / NexteraXT
Sample 1
$1,515
$1,800
 $2,300 $2,750
  Each additional sample
$125
$125
 $145  $145
Takara SMARTer Stranded Total RNA Pico v2 Mammalian

Sample 1

$1,360
 $1,585  $2,070 $2,420
  Each additional sample
$110
$110
$130
$130
RNA QC- Agilent & NanoDrop


     
RNA NanoChip (up to 12 samples)
$169     $262
 
RNA PicoChip (up to 11 samples)
$173
    $268
 
Small RNA (up to 11 samples)
 $190     $295
 
DNA1000 Chip (up to 12 samples)
$171

  $265
 
HS DNA Chip (up to 11 samples)
$200

   $310  

* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively. 

Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples)  ≥ 100ng/uL
PicoChip (11 samples)  ≤ 100ng/uL


Pricing listed above effective 4/1/2020.

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above.  This surcharge is subject to change.

Illumina service pricing is broken into two components: library preparation and sequencing.  Library preparation is charged on a per sample basis and varies by type.  Sequencing charges are based upon sequencing type (i.e.shared  2x150bp, 1x100bp, etc.)  and depth. 

Prices are subject to change due to increasing reagent costs.

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.