In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.
TruSeq Small RNA
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA / Nextera XT (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
T Cell Receptor α/β Profiling Kit
B Cell Receptor IgG H/K/L Profiling Kit
Agilent Sure Select Target Enrichment
We request all new users of our services please contact email@example.com to set up a meeting to discuss the proposed project.
To discuss a project or obtain a quote please e-mail firstname.lastname@example.org.
Please contact email@example.com to arrange all sample submissions.
All sample submissions must included an electronic (firstname.lastname@example.org) hard copy of the RNA Seq Submission form and an EXCEL sheet with each sample name, concentration and volume. Samples and sample submission forms may be submitted to the Gene Expression Center in room 3240 Biotech Center.
Illumina RNA-Seq Fillable Submission Form v11
RNA Sample Submission Guidelines Dec2019
Support is available for projects that are run at UWBC facilities, and is provided for a fee by the UWBC Bioinformatics Resource Center. Terminals and software are available in our computer lab for clients of UWBC facilities who are internal to UW-Madison or the University of Wisconsin System who need to perform further analysis but lack the necessary hardware and software.
A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement: "The author(s) thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing Illumina RNA library preparation and the DNA Sequencing Facility sequencing services”.
* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively.
Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples) ≥ 100ng/uL
PicoChip (11 samples) ≤ 100ng/uL
Pricing listed above effective 7/1/19.
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run, whether it is single or paired end and depth. Prices are subject to change due to increasing reagent costs.