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University of Wisconsin–Madison

Gene Expression Center


RNA Library Preparations Services

In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.

The following RNA library preparation services are available:

TruSeq Small RNA
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA  / Nextera XT  (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
T Cell Receptor α/β Profiling Kit
B Cell Receptor IgG H/K/L Profiling Kit

Agilent Sure Select Target Enrichment

We request all new users of our services please contact gecinfo@biotech.wisc.edu to set up a meeting to discuss the proposed project.

Getting Started

To discuss a project or obtain a quote please e-mail gecinfo@biotech.wisc.edu.

Sample Submission

Please contact gecinfo@biotech.wisc.edu to arrange all sample submissions.
All sample submissions must included an electronic (gecinfo@biotech.wisc.edu) hard copy of the RNA Seq Submission form and an EXCEL sheet with each sample name, concentration and volume.  Samples and sample submission forms may be submitted to the Gene Expression Center in room 3240 Biotech Center.

DOCUMENTS FOR DOWNLOAD

Illumina RNA-Seq Fillable Submission Form v11

RNA Sample Submission Guidelines Dec2019

Bioinformatics

Support is available for projects that are run at UWBC facilities, and is provided for a fee by the UWBC Bioinformatics Resource Center. Terminals and software are available in our computer lab for clients of UWBC facilities who are internal to UW-Madison or the University of Wisconsin System who need to perform further analysis but lack the necessary hardware and software.

Acknowledging UWBC and the Gene Expression Center

A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement: "The author(s) thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing Illumina RNA library preparation and the DNA Sequencing Facility sequencing services”.

  • GCW News

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    UW System
Rates

Non UW System Rates
Library Type  Details
Samples
1-48

Samples 
  49-96

Samples 
1-48

Samples
49-96

TruSeq Stranded mRNA   Sample 1
$1,435 $1,720
$2,185 $2,500
 
Each additional sample
$110 $110
$130 $130
TruSeq Stranded Total RNA Sample 1
 $1,515 $1,800
 $2,170 $2,590
  Each additional sample
  $190
$190
 $220 $220
    Samples 
1-24

Samples
  25-48

Samples
1-24

Samples  25-48
QiaSeq miRNA
Sample 1
$1,200
 $1,410 $1,790
$2,115
  Each additional sample
$180
$180
 $210 $210
    Samples  1-48
Samples
  49-96

Samples
1-48
 
Samples 
  49-96

Clontech SMARTer v4 / NexteraXT
Sample 1
$1,305
$1,555
 $1,960 $2,340
  Each additional sample
$105
$105
 $125  $125
Clontech SMARTer Stranded Total RNA Pico v2 Mammalian

Sample 1

$1,325
 $1,560  $1,820 $2,350
 




RNA QC- Agilent & NanoDrop


     
RNA NanoChip (up to 12 samples)
$155     $220
 
RNA PicoChip (up to 11 samples)
$159
    $225
 
DNA1000 Chip (up to 12 samples)
$157

  $225
 
HS DNA Chip (up to 11 samples)
$190

   $260  

* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively. 

Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples)  ≥ 100ng/uL
PicoChip (11 samples)  ≤ 100ng/uL


Pricing listed above effective 7/1/19.

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run, whether it is single or paired end and depth.  Prices are subject to change due to increasing reagent costs.

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.