In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina HiSeq2500 and PacBio Sequal platform.
TruSeq Small RNA
TruSeq Stranded Total & mRNA
TruSeq RNA v2
SMART-Seq v4 / Nextera XT (ultra-low input)
Agilent Sure Select Target Enrichment
The next gen seq team requests all new users of our services please contact firstname.lastname@example.org to set up a meeting to discuss the proposed project.
RNA Sample Submission Guidelines Jan2019
RNA-Seq Fillable Illumina Submission Form_v10
* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively. Agilent RNA chip type is dependent on concentration of RNA sample- >100ng/ul NanoChip (12 samples) & <100ng/ul PicoChip (11 samples).
Pricing listed above will be effective starting April 1, 2018.
EXTERNAL RATES: Clients not affiliated with UW-Madison or the University of Wisconsin system are charged a 53% surcharge for all labor provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.