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University of Wisconsin–Madison

Gene Expression Center

Illumina RNA Library Preparation Services

In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.

The GEC offers library preparation services for the following assays:

TruSeq Small RNA
TruSeq Stranded Total & mRNA
SMART-Seq® v4 Ultra® Low Input RNA  / Nextera XT  (ultra-low input)
SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian
T Cell Receptor α/β Profiling Kit
B Cell Receptor IgG H/K/L Profiling Kit

Agilent Sure Select Target Enrichment

The next gen seq team requests all new users of our services please contact nextgen-seq@biotech.wisc.edu to set up a meeting to discuss the proposed project.

RNA Sample Recommendations

• TruSeq Stranded mRNA & Total RNA Library Preps optimized for 100-1000ng total RNA

• SMART-Seq v4/ NexteraXT & Stranded Total RNA v2- Low Input Preps optimized for 1-10ng total RNA (RNA suspended in nuclease-free H2O)

• RNA samples should be DNAse treated

• A260/A280 ratio should be 1.8-2.1


DOCUMENTS FOR DOWNLOAD

RNA Sample Submission Guidelines Jan2019

RNA-Seq Fillable Illumina Submission Form20181101 v10
    UW System
Rates

External Rates
Library Type  Details
Samples
1-48

Samples 
>48

Samples 
1-48

Samples
>48

TruSeq Stranded mRNA   Sample 1
$1,435 $1,720
$2,185 $2,625
 
Each additional sample
$110 $110
$127 $127
TruSeq Stranded Total RNA Sample 1
 $1,515 $1,800
 $2,275 $2,715
  Each additional sample
  $190
$190
 $220 $220
    Samples 
1-16

Samples
17-32

Samples
1-16

Samples  
17-32

TruSeq Small RNA
Sample 1
$1,560
 $1,940 $2,327
$2,908
  Each additional sample
$160
$160
 $185 $185
    Samples  
1-48

Samples
>48

Samples
1-48
 
Samples 
>48

Clontech SMARTer v4 / NexteraXT
Sample 1
$1,305
$1,555
 $1,737 $1,985
  Each additional sample
$105
$105
 $121  $121
Clontech SMARTer Stranded Total RNA
Pico v2 Mammalian

Sample 1

$1,325
 $1,625  $1,995 $2,460
  Each additional sample
$150
$150
$173
$173
     Samples 
1-16

  Samples 
1-16
 
 
Ribo-Zero rRNA Reduction
Sample 1
$481
  $696
 
  Each additional sample
 $106    $122  
Agilent Bioanalyzer & NanoDrop Sample QC


     
RNA NanoChip
Samples 1-12   $155   $220
 
RNA PicoChip
Samples 1-11
 $159   $225
 
DNA1000 Chip
Samples 1-12
$157
  $225
 
HS DNA Chip
Samples 1-11
$168
   $260  

* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively. 

Agilent RNA chip type is dependent on concentration of RNA-
NanoChip (12 samples)  ≥ 100ng/uL
PicoChip (11 samples)  ≤ 100ng/uL

Pricing listed above effective 7/1/19.

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run, whether it is single or paired end and depth.  Prices are subject to change due to increasing reagent costs.

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.