In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina HiSeq2500 and PacBio Sequal platform.

The GEC offers library preparation services for the following assays:

TruSeq Small RNA
TruSeq Stranded Total & mRNA
TruSeq RNA v2
SMART-Seq v4  / Nextera XT  (ultra-low input)

Agilent Sure Select Target Enrichment

Iso-Seq RNA

Documents for download
RNA Submission Guidelines 20140930


RNA Sequencing Submission Form v7


Services



    UW System Rates
External Rates
Library Type  Details
Samples
1-48

Samples 
>48

Samples 
1-48

Samples
>48

TruSeq Stranded mRNA   Sample 1
$1,240 $1,435
$1,864 $2,064
 
Each additional sample
$90 $90
$104 $104
TruSeq Stranded Total RNA Sample 1
 $1,310 $1,510
 $1,945 $2,250
  Each additional sample
 
$160
 $185 $185
    Samples 
1-16

Samples
17-32

Samples
1-16

Samples  
17-32

TruSeq Small RNA
Sample 1
$1,560
 $1,940 $2,327
$2,908
  Each additional sample
$160
$160
 $185 $185
    Samples  
1-48

Samples
>48

Samples
1-48
 
Samples 
>48

Clontech SMARTer v4 / NexteraXT
Sample 1
$1,155
$1,380
 $1,737 $2,081
  Each additional sample
$80
$80
 $92  $92
Clontech SMARTer Stranded Total RNA
Pico v2 Mammalian

Sample 1

$1,155
 $1,300  $1,645 $2,104
  Each additional sample
$100
$100
$115
$115
     Samples 
1-16

  Samples 
1-16
 
 
Ribo-Zero rRNA Reduction
Sample 1
$481
  $696
 
  Each additional sample
 $106    $122  
Agilent Bioanalyzer & NanoDrop Sample QC


     
RNA NanoChip
Samples 1-12   $141   $216
 
RNA PicoChip
Samples 1-11
 $144   $220
 
DNA1000 Chip
Samples 1-12
$138
  $211
 
HS DNA Chip
Samples 1-11
$168
   $257  

* RNA is verified for quantity/purity and quality on a NanoDrop One Spectrophotometer and Agilent 2100 Bioanalyzer, respectively.  Agilent RNA chip type is dependent on concentration of RNA sample- >100ng/ul NanoChip (12 samples) & <100ng/ul PicoChip (11 samples).

Pricing listed above will be effective starting April 1, 2018.

EXTERNAL RATES: Clients not affiliated with UW-Madison or the University of Wisconsin system are charged a 53% surcharge for all labor provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation and sequencing cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run and whether it is single or paired end.  Prices are subject to change due to increasing reagent costs.

The next gen seq team requests all new users of our services to please contact nextgen-seq@biotech.wisc.edu to set up a meeting to discuss the proposed project.


Policy Statement

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.