The 10x Genomics Chromium Single Cell Gene Expression Assay combines microfluidics with molecular barcoding to provide digital 3’ mRNA quantification from thousands of individual single cells. The Single Cell Gene Expression Assay is able to generate single cell transcriptomic measurements from up to 10,000 cells per cell suspension with up to 65% capture rate efficiency enabling the identification of rare cell types from heterogeneous populations. User provided single cell or single nuclei suspensions are loaded onto the Chromium Controller to generate single cell + barcoded gel-bead emulsions from which Illumina-compatible library preps are generated and sequenced on the NovaSeq in collaboration with UWBC DNA Sequencing Facility followed by optional analysis using a custom developed single cell data analysis pipeline by the UWBC Bioinformatics Resource Center. The resulting data can then by analyzed and explored using the freely downloadable Loupe Cell Browser software (see support.10xgenomics.com/single-cell-gene-expression/software/visualization/latest/tutorial for Loupe Cell Browser free download, tutorial, and sample data sets).
Additionally, 10x Genomics offers assays for single cell immune profiling (sc-V(D)J enrichment kit), single cell copy number variation profiling, single cell chromatin accessibility analysis (sc-ATAC-Seq), de novo assembly, and genome or exome sequencing.
Documents for download: scRNA-Seq Fillable Submission Form_v5
Please e-mail firstname.lastname@example.org to schedule a meeting to discuss your proposed experiment and timeline!
10x Genomics Single Cell Features
Single Cell Suspension Submission Guidelines