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University of Wisconsin–Madison

Mass Spectrometry / Proteomics Facility

To our prospective clients - UW Biotechnology Center staff are currently working at home until further notice per University guidelines regarding COVID-19. We ask that you refrain from sending or dropping off samples until further notice. We apologize for the delay in service.
 
To our current clients - Thank you for your patience as we work to complete your projects. Expect delays of 2 or more weeks as we follow University guidelines regarding COVID-19.

Mass Spectrometry facility

See a presentation by the director of this facility here: Bio-Tech Talks

The University of Wisconsin Biotechnology Center has acquired several mass spectrometers for the analysis of biomolecules, including proteins, peptides, oligonucleotides, oligosaccharides and other small organic and inorganic molecules. In addition to measuring the molecular weight of compounds, the instruments are designed to perform MS/MS so that the structure of a selected ion can be deduced from the fragments. This is particularly useful for peptide sequencing and post-translational modification mapping. The ESI-MS are equipped with HPLC systems which are used with reversed phase (RP) nanobore / capillary columns, for LC/MS/MS experiments.

LC/MS/MS is extremely useful for quantitation of known compounds and for the rapid screening of a mixture to identify the components of interest. We also perform proteolytic digestions of proteins, which can be further analyzed by HPLC, MS, LC/MS and MS/MS for peptide mapping and peptide sequencing.

Equipment in the facility was purchased with funds from the University of Wisconsin, the National Institutes of Health (P50 GM64598, R33 DK070297),and the National Science Foundation (DBI-0520825, DBI-9977525)