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University of Wisconsin–Madison

Mass Spectrometry / Proteomics Facility

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

Learn about the Mass Spectrometry Core Facility and our new lipidomics platform
in this presentation by Facility Director Greg Barrett-Wilt.

Bio-Tech Talks

The University of Wisconsin Biotechnology Center Mass Spectrometry Core Facility has several mass spectrometers for the analysis of biomolecules, including proteins, peptides, oligonucleotides, oligosaccharides and other small organic and inorganic molecules. In addition to measuring the molecular weights of compounds, many of these instruments can perform MS/MS fragmentation data, so that the structure of a selected compound can be deduced from its fragments. This is particularly useful for peptide sequencing and post-translational modification mapping. The electrospray ionization (ESI) instruments are equipped with HPLC systems which can be used with reversed phase (RP) or hydrophilic-interaction (HILIC) chromatographies to better characterize complex mixtures by  LC/MS and LC/MS/MS.

LC/MS/MS is extremely useful for quantitation of known compounds and for the rapid screening of mixtures for profiling and to identify components of interest. We also perform proteolytic digestions of proteins, which can be analyzed by HPLC, MS, LC/MS and LC/MS/MS for peptide mapping,  peptide sequencing, and quantification.

The UWBC Mass Spectrometry Core Facility accepts samples from all Schools, Colleges, and Departments on the UW-Madison campus, across the University of Wisconsin System, academic institutions around the country, and commercial/industry clients around the world.  Let us know how we can help you!

Equipment in the facility was purchased with funds from the University of Wisconsin, the National Institutes of Health (P50 GM64598, R33 DK070297),and the National Science Foundation (DBI-0520825, DBI-9977525)