Learn about the Mass Spectrometry Core Facility and our new lipidomics platform
in this presentation by Facility Director Greg Barrett-Wilt.
of Wisconsin Biotechnology Center Mass Spectrometry Core Facility has several mass spectrometers
for the analysis of biomolecules, including proteins, peptides,
oligonucleotides, oligosaccharides and other small organic and inorganic
molecules. In addition to measuring the molecular weights of compounds, many of these instruments can perform MS/MS fragmentation data, so that the structure of
a selected compound can be deduced from its fragments. This is particularly
useful for peptide sequencing and post-translational modification mapping.
The electrospray ionization (ESI) instruments are equipped with HPLC systems which can be used with reversed
phase (RP) or hydrophilic-interaction (HILIC) chromatographies to better characterize complex mixtures by LC/MS and LC/MS/MS.
is extremely useful for quantitation of known compounds and for the rapid
screening of mixtures for profiling and to identify components of interest. We also
perform proteolytic digestions of proteins, which can be analyzed
by HPLC, MS, LC/MS and LC/MS/MS for peptide mapping, peptide sequencing, and quantification.
The UWBC Mass Spectrometry Core Facility accepts samples from all Schools, Colleges, and Departments on the UW-Madison campus, across the University of Wisconsin System, academic institutions around the country, and commercial/industry clients around the world. Let us know how we can help you!
Equipment in the facility was purchased with funds from the University of Wisconsin, the National Institutes of Health (P50 GM64598, R33 DK070297),and the National Science Foundation (DBI-0520825, DBI-9977525)