How can I use CRISPR?

CRISPR/Cas9 genome editing provides a highly reliable, efficient, and specific strategy to make customized changes to any targeted region of the genome. The unique, strategic advantage of CRISPR is the ease with which it is programmed to target your genomic region of choice. By synthesizing a single guide RNA (gRNA), we can direct the Cas9 protein to cut genomic DNA at this location. We can then harness the cell's DNA repair mechanisms to generate the edit we have designed during the DNA repair process.

Scissors cutting DNA

What kinds of edits can I make using CRISPR?

CRISPR cartoons.editsA wide range of edits are available to you once you have targeted the system to your region of interest.The most commonly desired edits including gene knockout, replacing several nucleotides, and large reporter knock-ins. If your desired type of edit is not immediately obvious, the specific strategy you choose will be determined by factors such as the experimental questions you wish to address, edit efficiency, and timeline.

What's in store for CRISPR?

Since the development of the CRISPR/Cas9 system, there has been a consistent stream of optimizations and enhancements of Cas9 protein, as well identification of alternative proteins with complementary features. Enhancements of the CRISPR system include maximizing specificity and efficiency, adding access to additional target sites, and expanding the use of CRISPR into functional genomics.