Welcome to the Sussman Lab

Crystal structure of AHA2

AHA2 from PDB rendered in PyMol

Research in the Sussman lab at the University of Wisconsin-Madison Biotechnology Center focuses on two main goals: (1) Development of novel technologies for global characterization of biological systems, and (2) Application of traditional genetics and biochemistry as well as novel technology to better understand the role of plasma membrane proteins in eukaryotic cell differentiation, with emphasis on the two model eukaryotes, Arabidopsis and yeast.

Within the realm of technology development, the laboratory has previously pioneered the development of a reverse genetic approach for performing functional genomics in Arabidopsis, as well as the development and use of new technologies for elucidating the downstream signaling components of plasma membrane hormone receptors. Transcriptome measurements using custom DNA tiling chips fabricated on a maskless array synthesizer (Nimblegen) have identified clusters of genes whose changes in expression are correlated with particular hormone response pathways initiated at the plasma membrane. Since 1998, the Sussman laboratory has been implementing various strategies for isotope-assisted mass spectrometry-based techniques to monitor changes in protein and metabolite expression as a compliment to the genomic technologies.

Researchers in the Sussman lab work on a wide range of projects within these broadly defined scopes. For more information on specific research topics, see the Research page.